The toys are mistakenly delivered to a day-care center instead of the attic right before Andy leaves for college, and it's up to Woody to convince the other toys that they weren't abandoned and to return home. Woody, Buzz and the whole gang are back. As their owner Andy prepares to depart for college, his loyal toys find themselves in daycare where untamed tots with their sticky little fingers do not play nice. So, it's all for one and one for all as they join Barbie's counterpart Ken, a thespian hedgehog named Mr. Pricklepants and a pink, strawberry-scented teddy bear called Lots-o'-Huggin' Bear to plan their great escape.

After his son is captured in the Great Barrier Reef and taken to Sydney, a timid clownfish sets out on a journey to bring him home. A clown fish named Marlin lives in the Great Barrier Reef loses his son, Nemo. After he ventures into the open sea, despite his father's constant warnings about many of the ocean's dangers. Nemo is abducted by a boat and netted up and sent to a dentist's office in Sydney. So, while Marlin ventures off to try to retrieve Nemo, Marlin meets a fish named Dory, a blue tang suffering from short-term memory loss. The companions travel a great distance, encountering various dangerous sea creatures such as sharks, anglerfish and jellyfish, in order to rescue Nemo from the dentist's office, which is situated by Sydney Harbor. While the two are doing this, Nemo and the other sea animals in the dentist's fish tank plot a way to return to Sydney Harbor to live their lives free again.